褰遍煶涓撳尯

 
    ISRFG 9th - Rice OneArray® introduction
 
絎節灞?ISRFG 浼氳鏈熼棿涓懼姙 Rice OneArray workshop錛屽崕鑱旀€葷粡鐞嗘潕鍦e鍗氬+浜茶嚜浠嬬粛鍗庤仈姘寸ɑ鑺墖 Rice OneArray 銆?
 
    琛ㄨ揪璋辨嫻嬫湇鍔?- 閫佹牱鏂規硶
 
鍗庤仈鐢熸妧鎻愪緵銆岃〃杈捐氨媯€嫻嬫湇鍔°€? 姝ゆ湇鍔℃槸鍒╃敤 OneArray® 鍏ㄥ熀鍥犺〃杈捐氨鑺墖媯€嫻?RNA 鏍峰搧 ( total RNA ) 涓鎭?RNA (mRNA) 琛ㄧ幇銆傛湰褰辯墖浠嬬粛濡備綍鍑嗗鍙婂瘎閫佹牱鍝併€?
 
    琛ㄨ揪璋辨嫻嬫湇鍔?- Overview
 
銆岃〃杈捐氨媯€嫻嬫湇鍔°€?鏄埄鐢?OneArray® 鍏ㄥ熀鍥犺〃杈捐氨鑺墖媯€嫻?RNA 鏍峰搧 ( total RNA ) 涓鎭?RNA (mRNA) 鐨勮〃鐜般€?瀹為獙嫻佺▼鏄牴鎹?1990 騫?Eberwine 瀹為獙瀹ょ殑鏂規硶鍔犱互鏀硅壇榪涜涓€榪炰覆瀹為獙鎿嶄綔涓庢暟鎹垎鏋愩€?
 
    琛ㄨ揪璋辨嫻嬫湇鍔℃祦紼?- (1) RNA QC
 
鏈郴鍒楀獎鐗囦負琛ㄨ揪璋辨嫻嬭緇嗘祦紼嬶紝鍏卞垎 5 闃舵錛?font class="line_hight_2_English">(1) RNA QC, (2) Target Preparation, (3) Target Labeling, (4) Hybridization, (5) Data Analysis銆?
 
    琛ㄨ揪璋辨嫻嬫湇鍔℃祦紼?- (2) Target Preparation
 
鏈郴鍒楀獎鐗囦負琛ㄨ揪璋辨嫻嬭緇嗘祦紼嬶紝鍏卞垎 5 闃舵錛?font class="line_hight_2_English">(1) RNA QC, (2) Target Preparation, (3) Target Labeling, (4) Hybridization, (5) Data Analysis銆?
 
    琛ㄨ揪璋辨嫻嬫湇鍔℃祦紼?- (3) Target Labeling
 
鏈郴鍒楀獎鐗囦負琛ㄨ揪璋辨嫻嬭緇嗘祦紼嬶紝鍏卞垎 5 闃舵錛?font class="line_hight_2_English">(1) RNA QC, (2) Target Preparation, (3) Target Labeling, (4) Hybridization, (5) Data Analysis銆?
 
    琛ㄨ揪璋辨嫻嬫湇鍔℃祦紼?- (4) Hybridization
 
鏈郴鍒楀獎鐗囦負琛ㄨ揪璋辨嫻嬭緇嗘祦紼嬶紝鍏卞垎 5 闃舵錛?font class="line_hight_2_English">(1) RNA QC, (2) Target Preparation, (3) Target Labeling, (4) Hybridization, (5) Data Analysis銆?
 
    琛ㄨ揪璋辨嫻嬫湇鍔℃祦紼?- (5) Data Analysis
 
鏈郴鍒楀獎鐗囦負琛ㄨ揪璋辨嫻嬭緇嗘祦紼嬶紝鍏卞垎 5 闃舵錛?font class="line_hight_2_English">(1) RNA QC, (2) Target Preparation, (3) Target Labeling, (4) Hybridization, (5) Data Analysis銆?
 
    OneArray® Protocol
 
鍗庤仈鍩哄洜鑺墖浜у搧 OneArray® 緋誨垪錛屽寘鎷〃杈捐氨鑺墖錛堜漢綾匯€佸ぇ榧犮€佸皬榧犮€佹按紼諱笌閰墊瘝鑿岀瓑鐗╃錛夊強寰皬 RNA 鑺墖錛堜漢綾匯€佸ぇ榧犲強灝忛紶銆佷笌閲嶈妯″紡緇忔祹浣滅墿錛夈€?
 
    Health Heroes (Discovery Channel)
 
鍗庤仈鐢熸妧鎺ュ彈Discovery棰戦亾涓撹錛屾挱鍑轟簬Health Heroes 緋誨垪鍗曞厓涔嬩腑銆?
 
    涓繪垚浠藉垎鏋?(PCA) 鏁欏
 
涓繪垚浠藉垎鏋?Principal Component Analysis錛孭CA)涓鴻姱鐗囨暟鎹垎鏋愮殑絎竴鍏籌紝灝嗚姱鐗囨暟涓囩偣鐨勪俊鎭畝鍖栨垚鍑犱釜浣庣淮搴︾殑鍒嗘瀽鏁版嵁錛屼互褰掔撼鍑哄郊姝ょ殑緹よ惤鍒嗗竷錛岃棄浠ユ瘮瀵瑰悇鑷殑榪戜技鍏崇郴錛涜瀵熸牱鏈棿鐨勬妧鏈€ф垨鐢熺墿鎬ч噸澶嶅仛鐨勫ソ涓嶅ソ銆佸彲涓嶅彲淇★紝榪涜€屽喅瀹氭槸鍚︾戶緇帴涓嬫潵鐨勮繘闃跺垎鏋愩€?
 
    鍗庤仈鐢熸妧- 鍏徃浠嬬粛
 

 
    Cytoscape 鏁欏
 
Cytoscape涓轟竴涓紑鏀炬簮鐮佺殑鐢熺墿淇℃伅杞歡錛屽畠鍙互寤烘瀯鍙鍖栫殑鍒嗗瓙浜や簰浣滅敤緗戠粶錛屽茍鍙皢宸叉湁鐨勫熀鍥犺〃杈句俊鎭?gene expression profiles) 鏁村悎榪涙緗戠粶涓紝杞繪槗瑙傚療鍒嗗瓙闂?(铔嬬櫧璐ㄢ€旇泲鐧借川 鎴?铔嬬櫧璐ㄢ€擠NA鈥? 鐨勫叧鑱旀€с€?
 
    鐜畤鐢靛彴銆愬垱鏂?00銆戜笓璁?/font>
 
2013/8/6 鍗庤仈鐢熸妧浣曟€葷粡鐞嗗彈閭€鑷崇幆瀹囩數鍙般€愬垱鏂?00銆戣妭鐩垎浜崕鑱旀煋鑹蹭綋鑺墖鐨勫簲鐢?
 
    澶х埍鐢佃鍙頒笓璁垮崕鑱旂敓鎶€
 
澶х埍鐢佃鍙般€屽彂鐜般€嶈妭鐩埗鎾柊绔逛笓棰橈紝鐗瑰湴鍓嶆潵鍗庤仈鐢熸妧涓撹錛屼簡瑙f渶鏂扮敓鎶€浜т笟瓚嬪娍
 

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