縐戠爺鏈嶅姟 > 絎簩浠e畾搴?> miRNA 鐮旂┒

 
榪愮敤絎簩浠e畾搴?(Next generation sequencing, NGS) 鎴栫О涓洪珮閫氶噺嫻嬪簭 (High throughput sequencing) 鐨勬妧鏈紝 闄ゅ畾搴忓錛岃繕鑳借兘綺懼噯鐨勮繘琛?miRNA 瀹氶噺錛? 鍔犻€熸柊 miRNA 鐨勫彂鐜幫紝 涓哄綋鍓嶇爺絀?miRNA 鏈€鏈夊姏鐨勫伐鍏楓€? 鍗庤仈鐢熸妧涓庣煡鍚嶇殑 miRNA 鍥㈤槦鍚堜綔錛屼互涓撲笟鐨勭殑緇忛獙錛? 鎻愪緵瀹㈡埛鍙︿竴涓喘鏂扮殑鏈嶅姟閫夋嫨銆?
 
 
   鏈哄瀷 / 杞綋
  • Illumina Genome Analyzer IIx
  • ACGT101-miR* 鍒嗘瀽杞綋

  • * Li, M. et al. MicroRNAome of porcine pre- and postnatal development. PLoS One 5, e11541.
       鎶ュ憡鑼冧緥
    miRNA 瀹氬簭鎶ュ憡浼氫緷搴忓垪姣斿鐨勭粨鏋滐紝 鍒嗘垚 4 涓兢緇勮繘琛屾暣鐞嗭紝 鍖呭惈 miRNA 鐨勫簭鍒楀強鍏惰〃鐜伴噺銆? 鎵€鏈?isoform 鐨勬嫹璐濇暟銆? 瀵瑰簲鐨勫墠浣?錛?font class="line_hight_2_English">pre-miRNA 闀垮害)銆?font class="line_hight_2_English">CG%銆丮FEI 鍊鹼紙minimal free energy index) 涓庝簩綰х粨鏋勭畝鍥劇瓑錛? 浠ュ浘鎴栬〃鏍肩殑鏂瑰紡錛屾洿鐩磋鐨勫憟鐜板垎鏋愮殑緇撴灉銆?
    • Reads 鍒濇絳涢€?/font>

    <<鎸夋鍥劇墖鏀懼ぇ>>
    浠? mRNA 鐨勮〃杈炬嫻嬩負渚嬶紝 鍏堝弬鑰冨熀鍥犵殑 RefSeq ID 榪涜 SYBR Green 緋葷粺鐨勬帰閽堣璁″悗錛? 浠? universal reference RNA 涓烘牱鍝佹祴璇曚駭鐗╃殑涓撲竴鎬с€? 鍗曚竴娉㈠嘲琛ㄧず浠呮湁涓€涓? PCR 浜х墿錛屽鍙沖浘鎵€紺恒€?


    • miRNAs 鍒嗙兢

    <<鎸夋鍥劇墖鏀懼ぇ>>
    灝嗚祫鏂欐瘮瀵瑰埌 miRNAs錛? 鏍規嵁鐗╃淇濆畧鎬у垎涓哄洓緇勨埗
    Group 1錛歊eads 鍙瘮瀵瑰埌 miRBase 涓凡鐭ョ殑浜虹被鎴栧叾浠栧摵涔沖姩鐗╃殑鍓嶄綋 錛坧re-miRNAs錛?/font>錛? 騫朵笖鍓嶄綋鍙互榪涗竴姝ユ瘮瀵瑰埌浜虹殑鍩哄洜緇勶紱鎴栧彲姣斿鍒頒漢綾誨叾浠栭儴鍒嗙殑鍩哄洜緇勶紝騫朵笖鍦ㄥ熀鍥犵粍涓婂歡浼稿彲浠ュ艦鎴愬彂澶圭粨鏋?(鍙堝垎鎴? 1a錛?b錛?c 涓夊皬緇?錛?br /> Group 2錛歊eads 鍙瘮瀵瑰埌 miRBase 鍝轟鉤鍔ㄧ墿鍓嶄綋錛? 鍓嶄綋涓嶈兘榪涗竴姝ユ瘮瀵瑰埌浜虹被鐨勫熀鍥犵粍錛屽彲浠ユ瘮瀵瑰埌鍩哄洜緇勪笂錛屽歡浼哥殑鍩哄洜緇勫簭鍒楀彲浠ュ艦鎴愭柊鐨勫彂澶圭粨鏋勶紱
    Group 3錛歊eads 姣斿鍒板摵涔沖姩鐗╁墠浣擄紝鍓嶄綋涓嶈兘姣斿鍒頒漢綾誨熀鍥犵粍涓婏紝 鍚屾椂涔熸棤娉曞湪浜虹殑鍩哄洜緇勪笂褰㈡垚鏂扮殑鍙戝す緇撴瀯錛?br /> Group 4錛歊eads 鐩存帴姣斿鍒板畾搴忕墿縐嶅熀鍥犵粍涓婂茍涓斿湪鍩哄洜緇勪笂寤朵幾鍙互褰㈡垚鏂扮殑鍙戝す緇撴瀯 錛圧eads 鏈瘮瀵逛笂宸茬煡鐨? miRNAs錛?/font>銆?

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