縐戠爺鏈嶅姟 > RNA 钀冨彇

 
浠庢牱鍝佷腑钀冨彇 total RNA 鏄爺絀惰漿褰曚綋瀛︾殑璧風偣錛? 浣嗘槸鍥犱負 RNA 鏈夋槗闄嶈В鐨勭壒鎬э紝騫朵笉鏄瘡涓€涓爺絀跺崟浣嶉兘鍏峰鐩稿叧鐨勮澶囧強鎶€鏈€備負鍗忓姪瀹㈡埛璺ㄨ繃姝ら棬妲涳紝 鍗庤仈鐢熸妧鎻愪緵涓嶅悓鏉ユ簮鏍峰搧 total RNA 鐨勮悆鍙栨湇鍔★紝騫跺悓鏃舵彁渚涘搧璐ㄦ嫻嬫暟鎹紝鑺傜渷璇曢獙鐨勬椂闂村強浜哄姏鐨勬秷鑰椼€? 鏈嶅姟鏀舵牱鏍囧噯濡備笅錛?
 
  鏍峰搧鏉ユ簮
  鏈€浣庢敹鏍烽噺
  緇嗚優鏍峰搧   ~ 1 x 106 cell   
鎴?/font> 3 cm dish 涓?/font> T25 flask 鍏昏嚦 8-9 鍒嗘弧
  緇勭粐鏍峰搧*   ~ 0.5 cm3 鎴?/font> > 50 mg
  琛€娑叉牱鍝?   2.5 ~ 5 ml 琛€娑?/font>
* 鑷粍緇囨垨琛€娑蹭腑鎶藉彇 RNA 闇€鑰冮噺杈冨鐨勫洜鏁幫紝渚嬪鏍峰搧鏉ユ簮銆佸彇鏍鋒柟寮忋€佹牱鍝佷繚瀛樼瓑鍥犵礌閮戒細閫犳垚褰卞搷銆傚緩璁厛涓庡崕鑱旂殑涓撲笟鍥㈤槦媧借緇嗚妭錛岃繘琛屽墠鏈熺殑璇勪及錛岀‘璁ゆ牱鍝佸鐞嗘祦紼嬭兘欏哄埄瀹屾垚銆?/font>
 
 
 
  鍝佽川媯€楠?/font>
 
  • Total RNA 閲?                      (媯€嫻嬪彇寰楃殑 total RNA 閲?
  • OD260/OD280 鈮?1.8       (紜繚鏃犲ぇ閲忚泲鐧借川鐨勬畫鐣?
  • OD260/OD230 鈮?1.6       (紜繚鏃犲ぇ閲忕洂綾繪垨鏈夋満婧跺墏鐨勬畫鐣?
  • RIN 鍊?                                  (媯€嫻?total RNA 瀹屾暣鎬?
 
 
 
 

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