縐戠爺鏈嶅姟 > 瀹氶噺 PCR > miRNA qPCR

 
鍗庤仈鐢熸妧涓烘洿鍏ㄩ潰鐨勬湇鍔″鎴鳳紝浠ュ騫寸殑鑺墖鎺㈤拡璁捐緇忛獙涓哄熀紜€錛? 鍦ㄤ弗璋ㄧ殑鍝佽川鎺х涓嬶紝鎺ㄥ嚭 mRNA 鍙? miRNA 鐨勫畾閲? PCR (qPCR) 媯€嫻嬫湇鍔°€? 瀹㈡埛浠呴渶瑕佹彁渚? 0.5 渭g 浠ヤ笂鐨? RNA 鏍峰搧錛? 鍗沖彲榪涜 SYBR Green 鎴? TaqMan 緋葷粺鐨? qPCR 媯€嫻嬶紝 鎻愪緵涓庤姱鐗囨暟鎹浉浜掗獙璇佹渶鍙俊璧栫殑宸ュ叿銆?
 
 
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棣栧厛鍒╃敤microRNA鐗瑰畾鐨勬湭绔?緇勭⒈鍩轟簬stem-loop RT primer錛屽叾涓?Font class="line_hight_2_English">stem-loop RT primer鍖呭惈UPL21鎺㈤拡 緇撳悎鐨勫簭鍒?榛勮壊鍖哄煙)錛屾帴鐫€璁捐涓撲竴鎬ч珮鐨?Font class="line_hight_2_English">Forward primer瀹屽叏浜掕ˉ浜?Font class="line_hight_2_English">microRNA(綰㈣壊鍖哄煙)錛屼絾Primer 搴忓垪 (緇胯壊綆ご)鍓嶇闇€瑙嗘儏鍐靛姞鍏ラ儴鍒嗙⒈鍩轟互鍒╂彁楂?Font class="line_hight_2_English">Primer Tm錛岃€?Font class="line_hight_2_English">Reverse primer鍒欐槸璁捐浜?Font class="line_hight_2_English">stem-loop RT primer搴? 鍒楀唴(鍥哄畾涓嶅彉)銆?


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涓轟簡紜繚瀹為獙鐨勫搧璐紝 Taqman 緋葷粺鐨?mRNA 琛ㄨ揪 qPCR 媯€嫻嬶紝 鍗庤仈鍏嶈垂鎻愪緵涓€鑸父瑙佺殑鎺у埗鎺㈤拡渚涘鎴蜂嬌鐢紝鍖呭惈閫傜敤浜庝漢綾匯€佸皬榧犮€佸ぇ榧犵瓑鏍峰搧鐨?U6 鍩哄洜銆? 姝ゅ錛屽叾浠栫殑鎺у埗鍩哄洜鎺㈤拡錛屽垯闇€瑙嗗鎴風殑闇€姹傚彟澶栬璁″強鍚堟垚寮曞瓙銆?
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鍗庤仈鎶€鏈洟闃熶互 lung reference RNA 涓?brain reference RNA 涓烘祴璇曟牱鍝佽繘琛?UPL microRNA Taqman 嫻嬭瘯錛? 瀹為獙緇撴灉涓?MAQC 鐨?TaqMan qPCR 璧勬枡鍜屽強 OneArray®聽 浜虹被琛ㄨ揪璋辮姱鐗囨暟鎹繘琛岀浉浜掓瘮瀵癸紝 緇撴灉鏄劇ず涓夎€呴棿鍛堢幇楂樺害鐩稿叧鎬с€?


   鎶ュ憡鑼冧緥涓嬭澆
  • miRNA qPCR 鎶ュ憡鑼冧緥

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