縐戠爺鏈嶅姟 > 瀹氶噺 PCR > mRNA qPCR

 
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浠? mRNA 鐨勮〃杈炬嫻嬩負渚嬶紝 鍏堝弬鑰冨熀鍥犵殑 RefSeq ID 榪涜 SYBR Green 緋葷粺鐨勬帰閽堣璁″悗錛? 浠? universal reference RNA 涓烘牱鍝佹祴璇曚駭鐗╃殑涓撲竴鎬с€? 鍗曚竴娉㈠嘲琛ㄧず浠呮湁涓€涓? PCR 浜х墿錛屽鍙沖浘鎵€紺恒€?


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涓轟簡紜繚瀹為獙鐨勫搧璐紝 SYBR Green 緋葷粺鐨?mRNA 琛ㄨ揪 qPCR 媯€嫻嬶紝 鍗庤仈鍏嶈垂鎻愪緵涓€鑸父瑙佺殑鎺у埗鎺㈤拡渚涘鎴蜂嬌鐢紝鍖呭惈閫傜敤浜庝漢綾匯€佸皬榧犮€佸ぇ榧犵瓑鏍峰搧鐨?GAPDH 鍙?尾-actin 鍩哄洜鍙婇€傜敤浜庨叺姣嶈弻鐨?PDH1 鍙?ACT1 鍩哄洜銆? 姝ゅ錛屽叾浠栫殑鎺у埗鍩哄洜鎺㈤拡錛屽垯闇€瑙嗗鎴風殑闇€姹傚彟澶栬璁″強鍚堟垚寮曞瓙銆?
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鍗庤仈鎶€鏈洟闃熶互 universal reference RNA 涓?brain reference RNA 涓烘祴璇曟牱鍝佽繘琛?SYBR Green qPCR 嫻嬭瘯錛? 瀹為獙緇撴灉涓?MAQC 鐨?TaqMan qPCR 璧勬枡鍜屽強 OneArray®聽 浜虹被琛ㄨ揪璋辮姱鐗囨暟鎹繘琛岀浉浜掓瘮瀵癸紝 緇撴灉鏄劇ず涓夎€呴棿鍛堢幇楂樺害鐩稿叧鎬с€?


   鎶ュ憡鑼冧緥涓嬭澆
  • mRNA qPCR 鎶ュ憡鑼冧緥

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